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	Toxin Name Δ-miturgitoxin-Cp1f Source Species Cheiracanthium  punctorium Toxin Group Miturgitoxin Description Spider venom toxin that exhibits cytolytic activity by forming an alpha-helix across the membrane. Causes instant paralysis and death in the larvae of the flesh fly Sarcophaga carnaria at doses of 1326 pmol/g and reversible paralysis at doses of less than 663 pmol/g. Has cytolytic activity against insect Sf9 cells. Causes stable and irreversible depolarization of fly muscle fibers, leading to contracture at higher toxin concentrations. Destabilizes membranes. Discovered 2010 This toxin last updated on Sep 23, 2014 Taxonomy Current Taxonomy Historic Taxonomy Kingdom Animalia Phylum Arthropoda Class Arachnida Order Araneae Infra-order Araneomorphae Family Miturgidae Genus Cheiracanthium Species punctorium "Anyphaena" "nutrix" "Aranea" "nutrix" "Aranea" "punctoria" "Cheiracanthium" "italicum" "Cheiracanthium" "nutrix" "Cheiracanthium" "punctorium" "Clubiona" "nutrix" "Drassus" "maxillosus" Biological Activity Cytolytic Molecular Target ED50 IC50 Kd Pharmacophore Comment Cytolytic 5% toxicity at 0.5 uM and approximately 20% cell death at 10 uM Taxon ED50 LD50 PD50 Qualitative Information Sarcophaga carnaria 1326.0  pmol/g Converted based on the literature data of 20 μg/g (in larvae) and a molecular mass of 15034.12 g/mol. 663 pmol/g cause reversible paralysis. Accessions Source Accession ArachnoServer AS002084 EMBL KF155270 EMBL AHH30788 NCBI_Taxon 682790 UniProt TX1_CHEPU Literature References Original Deposition References Vassilevski A.A., Fedorova I.M., Maleeva E.E., Korolkova Y.V., Efimova S.S., Samsonova O.V., Schagina L.V., Feofanov A.V., Magazanik L.G., Grishin E.V. J. Biol. Chem. 285:32293-32302(2010). Novel class of spider toxin: active principle from the yellow sac spider Cheiracanthium punctorium venom is a unique two-domain polypeptide. Kuhn-Nentwig L., Fedorova I.M., Luscher B.P., Kopp L.S., Trachsel C., Schaller J., Vu X.L., Seebeck T., Streitberger K., Nentwig W., Sigel E., Magazanik L.G. J. Biol. Chem. 287:25640-25649(2012). A venom-derived neurotoxin, CsTx-1, from the spider Cupiennius salei exhibits cytolytic activities. Protein Information Disulfide Bonds Posttranslational modifications Left Residue Right Residue Evidence 4 19 Experimentally determined 11 28 Experimentally determined 18 41 Experimentally determined 30 39 Experimentally determined 68 83 Experimentally determined 75 92 Experimentally determined 82 110 Experimentally determined 94 108 Experimentally determined Residue Number Type Symbol 134 C-terminal amidation NH₂ Sequences Peptide Sequences >as:Δ-miturgitoxin-Cp1f|ENA:AHH30788 Translation of a cytolytic peptide from the spider Cheiracanthium punctorium MKFSLFFSVFFLAVLHACLSESEIDLEDEEHFMSSDSFLSEIQDESRGKTCIERSKECTN DRHGCCRGKIFKDKCTCVKSGKTEKCVCTQKKWAKIIESYIGDIPALPKPVDDKCVPKHA DCSKRKDDCCKGGIFKYQCKCYDMYDDDGEKTDLCGCVSPVEHQAIEGALRIAKKLIGDR WGR Full BLAST BLAST mature toxin only mRNA Sequences >as:Δ-miturgitoxin-Cp1f|ENA:KF155270 cDNA sequence of a cytolytic peptide from the spider Cheiracanthium punctorium AACGGTCTTCATATCTTCCCAAGGTGCTTGCTGGAGAATCAAGAGTTGGTGATTTGCCCT TCTTATATTTTACGATGAAGTTTTCGTTGTTCTTCAGCGTGTTTTTTCTGGCTGTCTTGC ACGCTTGCTTATCAGAAAGCGAGATAGACCTTGAAGATGAAGAGCACTTCATGAGCTCCG ACTCCTTTTTGAGTGAAATACAAGACGAATCAAGAGGAAAAACGTGTATTGAAAGGAGCA AGGAATGCACCAATGACCGCCACGGCTGTTGTCGAGGTAAAATATTCAAAGACAAATGCA CGTGCGTCAAAAGTGGTAAAACAGAAAAATGCGTTTGCACACAGAAGAAATGGGCCAAGA TCATTGAAAGTTACATTGGAGACATCCCAGCCTTGCCGAAACCCGTAGACGACAAATGCG TCCCGAAGCACGCCGACTGCAGCAAGCGGAAGGACGACTGCTGCAAGGGTGGGATCTTCA AGTACCAGTGCAAGTGCTACGACATGTATGACGATGATGGAGAGAAAACGGACTTGTGCG GCTGCGTTTCGCCAGTAGAGCACCAGGCCATCGAAGGGGCCCTGAGGATCGCAAAGAAGC TCATCGGCGACAGATGGGGCCGATGAATCCGCTGGTTTGGAGATA Full BLAST Toxin Synonyms Synonym Type Δ-miturgitoxin-Cp1f Recommended full name Δ-MGTX-Cp1f Recommended abbreviation CpTx1-1f Synonym
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